Officers say they were called on Saturday, November 17, at 11.10pm to a two car crash on the A515, Clifton Road, Ashbourne.
The incident involved a silver Mercedes C Class, travelling away from Ashbourne, and a white Renault Clio which was travelling in the opposite direction.
The driver of the Mercedes, a 49-year-old woman from Swadlincote, and the driver of the Renault, a 26-year-old woman from Hatton, were both pronounced dead at the scene.
A Derbyshire Constabulary Spokesperson said: "The families of both women have been made aware and our thoughts are with them at this time.
"Anyone who may have witnessed the crash or has dashcam footage of the incident or the moments preceding it - is asked to contact us using the below methods."
- Facebook – send a private message to /DerbyshireConstabulary
- Twitter – direct message the contact centre on @DerPolContact
- Website – complete the online contact form www.derbyshire.police.uk/Contact-Us.
- Phone – call 101.
"Please quote the reference number 18*557391 and the name of the officer in the case DC Siddall, in any correspondence.You can also call Crimestoppers anonymously on 0800 555 111."
In the event of an emergency you should always call 999.